吸光光度法（紫外/可见光谱）讲座（6）
<H2 style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; TEXT-ALIGN: center; mso-layout-grid-align: none" align=center><SPAN style="FONT-SIZE: 14pt; FONT-FAMILY: 黑体; mso-bidi-font-size: 16.0pt; mso-ascii-font-family: Arial; mso-font-kerning: 0pt">吸光光度法（紫外</SPAN><SPAN lang=EN-US style="FONT-SIZE: 14pt; mso-bidi-font-size: 16.0pt; mso-font-kerning: 0pt"><FONT face=Arial>/</FONT></SPAN><SPAN style="FONT-SIZE: 14pt; FONT-FAMILY: 黑体; mso-bidi-font-size: 16.0pt; mso-ascii-font-family: Arial; mso-font-kerning: 0pt">可见光谱）讲座（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 14pt; mso-bidi-font-size: 16.0pt; mso-font-kerning: 0pt"><FONT face=Arial>6</FONT></SPAN><SPAN style="FONT-SIZE: 14pt; FONT-FAMILY: 黑体; mso-bidi-font-size: 16.0pt; mso-ascii-font-family: Arial; mso-font-kerning: 0pt">）<FONT face=Arial><BR></FONT></SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 16.0pt; mso-font-kerning: 0pt">――</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 16.0pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">应用吸光光度法测定配合物的组成</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 16.0pt; mso-font-kerning: 0pt"><?xml:namespace prefix = o /><o:p></o:p></SPAN></H2>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"> <o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none"><B><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">2 </SPAN></B><B><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">平衡移动法</SPAN></B><B><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN></B>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">在一系列相同体积的容量瓶中，加入一定量的金属离子</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">M</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">，以及逐点增量的显色剂</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">R</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（保持</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">M</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">尚未全部形成配合物），显色定容使反应达到平衡，相对于各自的空白测量这一系列溶液的吸光度。在另一同体积容量瓶中，加入同样量</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">M</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">，过量的显色剂</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">R</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">，以便使</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">M</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">最大限度地被</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">R</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">配合，然后相对其空白测定最大吸光度</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>max</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">，以</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lg [A<SUB>i<SPAN style="mso-spacerun: yes"> </SPAN></SUB>/</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>max</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">对</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lg[R]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">作图，所得直线斜率即为配合比。这种方法称为平衡移动法又叫做有限对数法。此法对离解度较大的配合物以及可形成几种配合物的体系，也能得到满意的结果，因此应用较广。</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">假设配合平衡为</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; TEXT-ALIGN: center; mso-layout-grid-align: none" align=center><SPAN lang=EN-US style="FONT-SIZE: 12pt; LETTER-SPACING: -0.6pt; mso-font-kerning: 0pt">M</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; LETTER-SPACING: -0.6pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; LETTER-SPACING: -0.6pt; mso-font-kerning: 0pt">nR </SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; LETTER-SPACING: -0.6pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN style="FONT-SIZE: 12pt; LETTER-SPACING: -0.6pt; mso-font-kerning: 0pt"> <SPAN lang=EN-US>MR<SUB>n</SUB></SPAN></SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; TEXT-ALIGN: center; mso-layout-grid-align: none" align=center><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">K</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">[MR<SUB>n</SUB>] /</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">[M][R]<SUP>n</SUP></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; TEXT-ALIGN: center; mso-layout-grid-align: none" align=center><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lg</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">[MR<SUB>n</SUB>] / [M]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lgK</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lg[R]<o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><SPAN style="mso-spacerun: yes"> </SPAN></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">因为</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><SPAN style="mso-spacerun: yes"> </SPAN>[MR<SUB>n</SUB>] / [M]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>i<SPAN style="mso-spacerun: yes"> </SPAN></SUB>/</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>max</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">所以</SPAN><SPAN style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"> <SPAN style="mso-spacerun: yes"> </SPAN><SPAN lang=EN-US>lg [A<SUB>i<SPAN style="mso-spacerun: yes"> </SPAN></SUB>/</SPAN></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>max</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lg[R]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lgK</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><SPAN style="mso-spacerun: yes"> </SPAN></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">1-32</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">用</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lg [A<SUB>i<SPAN style="mso-spacerun: yes"> </SPAN></SUB>/</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>max</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">对</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lg[R]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">作图，所得直线斜率即为</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">。当</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">l-32</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">式中</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lg [A<SUB>i<SPAN style="mso-spacerun: yes"> </SPAN></SUB>/</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>max</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">0</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">时，</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lgK</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝－</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">nlg[R]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">，因此，本法还可以求出配合物的表观稳定常数。</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">有时，所用显色剂的纯度并不知道，那么</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">[R]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">的精确值就难以确定，但这并不影响平衡移动法的使用，可以用加入显色剂溶液的体积</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">V<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">去代替</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">[R]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">。这可以通过简单的推导来说明。</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lg [A<SUB>i<SPAN style="mso-spacerun: yes"> </SPAN></SUB>/</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>max</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">A<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lg[R]</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lgK</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN></SUB>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><SPAN style="mso-spacerun: yes"> </SPAN></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lg</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">V<SUB>i</SUB>c</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">未</SPAN></SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">/V</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">测</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lgK</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><SPAN style="mso-spacerun: yes"> </SPAN></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lgV<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lg</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">c</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">未</SPAN></SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">/V</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">测</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lgK</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 144pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 12.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lgV<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">K</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">’</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">式中</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><SPAN style="mso-spacerun: yes"> </SPAN>V<SUB>i</SUB></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">――系列溶液各点加入显色剂溶液的体积；</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 36pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 3.0; mso-char-indent-size: 12.0pt"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">c</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">未</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">――尚未精确知道的显色剂溶液浓度；</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 36pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 3.0; mso-char-indent-size: 12.0pt"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">V</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">测</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">――显色测定溶液的体积；</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 36pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 3.0; mso-char-indent-size: 12.0pt"><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">K</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: Arial; mso-font-kerning: 0pt; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial">’</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">――</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">n lg</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">c</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">未</SPAN></SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">/V</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">测</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）＋</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">lgK</SPAN><SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">稳</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">。</SPAN>
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