吸光光度法（紫外/可见光谱）讲座（14）
<H3 style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; TEXT-ALIGN: center; mso-layout-grid-align: none" align=center><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 16.0pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">――提高吸光光度法灵敏度和选择性的某些途径</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 16.0pt; mso-font-kerning: 0pt"><?xml:namespace prefix = o /><o:p></o:p></SPAN></H3>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN lang=EN-US style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none"><B><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">5 </SPAN></B><B><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">萃取光度、浮选光度和固相光度法</SPAN></B><B><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN></B>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">这三类方法都是将待测组分先分离、富集到另一相后再进行光度测定，从而提高测定灵敏度和选择性。</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">1</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"> </SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">萃取光度法</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">把待测组分从一种液相（水相）转移到另一种液相（有机相），以达到分离和富集目的的过程称为萃取。萃取是基于溶质在两种基本不相混溶的溶剂之间的分配差异来实现分离的。只要溶质在有机溶剂中的溶解度大于在水中的溶解度就可能实现萃取。由于带电荷的化合物不能进入有机溶剂，金属离子必须转变为不带电荷的配合物，或与带相反电荷的离子形成离子（对）缔合物后才能被萃取入有机相。</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^; mso-font-width: 693%">萃取光度法就是将溶剂萃取与光度测定相结合的一种分析方法。它将水相中的有色</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">物质直接萃取到少量与水不互溶的溶剂中后进行吸光度测定，它特别适用于显色产物难溶于水的体系。由于萃取的分离和富集作用，能有效地提高测定的灵敏度和选择性。例如，用乙醚萃取</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">Mo</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">Ⅴ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）与</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"> SCN</SPAN><SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">形成的配合物后进行光度测定，比直接在水相中测定灵敏度提高九倍；利用</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">Pd</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">Ⅱ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）与</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">DDO</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（双十二烷基二硫代乙酸胺）二元配合物的萃取光度法测定钯，其</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">ε</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">高达</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">3.6</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">×</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">10<SUP>6</SUP> </SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">L</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">?</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">mol</SPAN><SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-font-kerning: 0pt">-1</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">?</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">cm</SPAN><SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-font-kerning: 0pt">-1</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">，是一种超高灵敏度的测定方法。</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">2</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"> </SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">浮选光度法</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">浮选光度法可分为溶剂浮选和泡沫浮选两类。前者靠有机溶剂将水相试液中的待测物带到两相界面上，经分离和除去溶剂后，再把待测物溶于少量的另一种溶剂中进行光度测定。例如，在［</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">H<SUP>+</SUP></SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">］＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">0.45 mol / L</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">介质中，用环己烷作浮选剂浮选</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"> Hg</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">Ⅱ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">-I</SPAN><SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN></SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">-</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">亚甲基蓝配合物使之与水相分离，蒸发除去环己烷，残余物溶于少量甲醇中后进行光度测定，</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体">ε<SUB><SPAN lang=EN-US>670</SPAN></SUB></SPAN><SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt">nm</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt">1.5</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^">×</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt">10<SUP>5</SUP></SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"> L</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">?</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">mol</SPAN><SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-font-kerning: 0pt">-1</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">?</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">cm</SPAN><SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-font-kerning: 0pt">-1</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">。泡沫浮选法则使用适当的起泡剂（如表面活性剂），使待测组分以离子形式或配合物形式随泡沫一起与原试样溶液分离，随后加入合适的消泡剂消泡后进行光度测定。例如在</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">pH7</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">的条件下，以</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">CTMAB</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">作起泡剂浮选</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">CN</SPAN><SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN></SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">-</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">氯胺</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">T-</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">异烟酸吡唑啉酮配合物，然后用乙醇作消泡剂，可用于</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">CN</SPAN><SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">－</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">的测定，</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体">ε<SUB><SPAN lang=EN-US>638</SPAN></SUB></SPAN><SUB><SPAN lang=EN-US style="FONT-SIZE: 12pt">nm</SPAN></SUB><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^">＝</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt">5</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^">×</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt">10<SUP>4</SUP></SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt"> L</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">?</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">mol</SPAN><SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-font-kerning: 0pt">-1</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">?</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-font-kerning: 0pt">cm</SPAN><SUP><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-font-kerning: 0pt">-1</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">。</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; LINE-HEIGHT: 150%; mso-layout-grid-align: none; mso-char-indent-count: 2.0; mso-char-indent-size: 12.0pt"><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">（</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt">3</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">）</SPAN><SPAN style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"> </SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-font-kerning: 0pt; mso-hansi-font-family: ^Times New Roman^">固相光度法</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt"><o:p></o:p></SPAN>
<SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 10.5pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">这是利用固相载体（离子交换树脂、泡沫塑料或滤纸）对待测组分进行分离、富集并显色，随后直接测定固相吸光度的方法。例如，已有强碱性阴离子交换树脂作载体，用于</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 10.5pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Bi</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">I<SUB>4</SUB></SPAN><SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">－</SPAN></SUP><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">，</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Bi</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Ⅲ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">-4</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">邻苯三酚红，</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Hg</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Ⅱ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">-</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">双碱腙和磷（砷）杂多蓝等显色体系；强酸性阳离子交换树脂作载体用于</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Nb</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Ⅴ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">-5-Br-PADAP-</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">酒石酸显色体系；泡沫塑料作载体用于</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Au</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Ⅲ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">-</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">硫代米黄酮，</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Zn</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">（</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Ⅱ</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">）</SPAN><SPAN lang=EN-US style="FONT-SIZE: 12pt; FONT-FAMILY: ^Times New Roman^; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">-</SPAN><SPAN style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-size: 9.0pt; mso-ascii-font-family: ^Times New Roman^; mso-hansi-font-family: ^Times New Roman^; mso-bidi-font-family: ^Times New Roman^; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">双硫腙等显色体系的报导。</SPAN>
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