洗板机使用范围(页 1) - 『实验室常规仪器』 - 生化分析仪器网【仪器论坛】

阳光发表于 2004-3-31 00:00

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自ELISA问世20多年来，洗板由原来的手工操作，发展到简单的多头加液器，再形成自动化的洗板机，随着时间的推移，逐步融合了冲洗压力、冲洗距离、冲洗时间、震荡、涡流、底部冲洗、两点吸液、连续式冲洗等多种方式，增加了多规格微孔形状的选择等。

一、洗板机机理的研究<o:p></o:p>

1、 快速的、与残留液量相关的直接稀释过程；

2、 受扩散影响、与时间相关的残留液层的间接稀释过程；

3、 优化洗液的运动可以降低残留层，改善洗涤的效率，降低背景变异，提高整个

试验的灵敏度；

4、 相同的振幅与频率对洗涤效果有影响；

5、 循环次数、浸泡时间、冲洗压力、冲洗时间对洗涤效果的影响在以上研究中，

有指导意义的是关于两个物理过程的分析，与残留量相关的直接稀释要求尽可能少的残留量，与反应相关的间接稀释，要求有一定的浸泡时间。另外，多次洗涤对效果有改进；底部冲洗由于距离近，冲力大，可改善洗涤效果。

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二、洗板机的发展状况<o:p></o:p>

洗板仪器的发展，可以大致分为三个阶段，简易型、自动型、集成环境式洗板机。

1、简易型也可称为手动式洗板器，一般由负压泵与清洗头可完成最基本的吸液，也有可完成加液动作的装置。这类装置，由于成本极低，使用方便，工作量不大时可以使用。

2、自动式洗板机：可根据用户的设置完成规定次数与条数的洗板工作，有的具有简单的存贮功能。主要目的是利用自动化技术，替代手工劳动。其中有条式与板式之分。如LABSYSTEM的W4、BIO-RAD早期的1250、拓普公司的DEM-2等。

3、集成环境式洗板机是随着ELISA的发展、由仪器生产厂商根据ELISA应用专家的建议，结合各自对洗板技术的研究而发展起来的，其特点在于提供良好的洗板环境，强调洗涤效果而非自动化，具有一定的智能特性，如LABSYSTEM的ACSENT WASH，拓普的DEM-3等。

4、洗板环境实际上是一组有利于改进洗涤效果的参数及洗涤动作。对于具体应用来说，有的很实用，效果也不错，如底部冲洗、两点吸液、震动、交叉吸液（漂洗）等；有的只有厂家的广告词而已。精心设计的洗板条件确实可以取得很好的效果。

5、符合中国ELISA应用具体情况的洗板机，除了洗涤彻底、稳定、可靠的要求外，还应具有：中文显示、操作简便、具有微孔板位置调节功能、配件方便且价格合理、售后服务良好，具有两点吸液、底部冲洗、交叉吸液等功能，可洗涤48孔板等。

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三、国内常见洗板机简介<o:p></o:p>

洗板，是PCR ELISA实验中重要的操作程序，是避免产物污染的关键步骤。另外，洗板的好坏，直接关系到实验的成败。在没有洗板机的情况下，PCR ELISA实验中洗板是整个实验中控制污染的重要环节，须十分小心操作。正确使用洗板机，采用相应的防污染措施可减少污染机会。

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中国市场常见的洗板机一览表<o:p></o:p>

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生产<o:p></o:p>

厂家<o:p></o:p>

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产品<o:p></o:p>

型号<o:p></o:p>

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适用<o:p></o:p>

微孔板<o:p></o:p>

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清洗<o:p></o:p>

头<o:p></o:p>

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加液量<o:p></o:p>

（uL）<o:p></o:p>

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残留量<o:p></o:p>

（uL）<o:p></o:p>

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浸泡<o:p></o:p>

时间<o:p></o:p>

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震<o:p></o:p>

动<o:p></o:p>

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两点<o:p></o:p>

吸液<o:p></o:p>

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底部<o:p></o:p>

冲洗<o:p></o:p>

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洗液<o:p></o:p>

种类<o:p></o:p>

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拓普<o:p></o:p>

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DEM-2<o:p></o:p>

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96/48<o:p></o:p>

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8/12<o:p></o:p>

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0-500<o:p></o:p>

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＜6<o:p></o:p>

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0-600s<o:p></o:p>

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无<o:p></o:p>

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无<o:p></o:p>

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有<o:p></o:p>

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1<o:p></o:p>

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拓普<o:p></o:p>

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DEM-3<o:p></o:p>

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96/48<o:p></o:p>

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8/12<o:p></o:p>

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0-500<o:p></o:p>

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＜2<o:p></o:p>

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0-240s<o:p></o:p>

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有<o:p></o:p>

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有<o:p></o:p>

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有<o:p></o:p>

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1<o:p></o:p>

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拓普<o:p></o:p>

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DEM-3A<o:p></o:p>

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96/48<o:p></o:p>

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8/12<o:p></o:p>

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50-3600<o:p></o:p>

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＜2<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 45.25pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=60>
0-240s<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 21pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=28>
有<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 34.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=46>
有<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=39>
有<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.7pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=40>
2<o:p></o:p>

</TD></TR>
<TR style="HEIGHT: 18.6pt">
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 0.5pt solid; WIDTH: 49.85pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-top-alt: solid windowtext .5pt" width=66>
上海雷勃<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 52.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=69>
Well wash4<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 38.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=51>
96<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 31.35pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=42>
8/12<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 40.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=54>
0-750<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 48pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=64>
＜5<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 45.25pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=60>
0-600s<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 21pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=28>
无<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 34.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=46>
无<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=39>
无<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.7pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=40>
1<o:p></o:p>

</TD></TR>
<TR style="HEIGHT: 19.5pt">
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 0.5pt solid; WIDTH: 49.85pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-top-alt: solid windowtext .5pt" width=66>
雷勃<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 52.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=69>
Well wash<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 38.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=51>
96<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 31.35pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=42>
8/12<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 40.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=54>
50-1000<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 48pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=64>
＜3standard<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 45.25pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=60>
1-3600s<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 21pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=28>
有<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 34.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=46>
Sweep<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=39>
无<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.7pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=40>
2<o:p></o:p>

</TD></TR>
<TR style="HEIGHT: 18.6pt">
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 0.5pt solid; WIDTH: 49.85pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-top-alt: solid windowtext .5pt" width=66>
Labsystem<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 52.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=69>
Ascent<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 38.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=51>
 <o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 31.35pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=42>
16/24<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 40.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=54>
 <o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 48pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=64>
＜1sweep<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 45.25pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=60>
 <o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 21pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=28>
 <o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 34.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=46>
 <o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=39>
 <o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.7pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=40>
 <o:p></o:p>

</TD></TR>
<TR style="HEIGHT: 19.5pt">
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 0.5pt solid; WIDTH: 49.85pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-top-alt: solid windowtext .5pt" width=66>
Bio-Rad<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 52.05pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=69>
1250<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 38.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=51>
96<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 31.35pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=42>
8/12<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 40.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=54>
50-400<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 48pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=64>
＜5<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 45.25pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=60>
0-250s<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 21pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=28>
无<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 34.8pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=46>
无<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.2pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=39>
无<o:p></o:p>

</TD>
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: medium none; WIDTH: 29.7pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 19.5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" width=40>
1<o:p></o:p>

</TD></TR>
<TR style="HEIGHT: 18.6pt">
<TD style="BORDER-RIGHT: windowtext 0.5pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: medium none; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: windowtext 0.5pt solid; WIDTH: 49.85pt; PADDING-TOP: 0cm; BORDER-BOTTOM: windowtext 0.5pt solid; HEIGHT: 18.6pt; mso-border-top-alt: solid windowtext .5pt" width=66>
Bio-Rad<o:p></o:p>

</TD>
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1575<o:p></o:p>

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96<o:p></o:p>

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8/12<o:p></o:p>

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50-3000<o:p></o:p>

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＜2<o:p></o:p>

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0-59Min<o:p></o:p>

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有<o:p></o:p>

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Sweep<o:p></o:p>

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有<o:p></o:p>

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1<o:p></o:p>

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Bio-Tek<o:p></o:p>

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Elx50<o:p></o:p>

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96<o:p></o:p>

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8/12<o:p></o:p>

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50-3000<o:p></o:p>

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＜5<o:p></o:p>

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0-600s<o:p></o:p>

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有<o:p></o:p>

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-<o:p></o:p>

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-<o:p></o:p>

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1<o:p></o:p>

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Awarenes<o:p></o:p>

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Fax2600<o:p></o:p>

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96<o:p></o:p>

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8/12<o:p></o:p>

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0-500<o:p></o:p>

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＜3<o:p></o:p>

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0-99Min<o:p></o:p>

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无<o:p></o:p>

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无<o:p></o:p>

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无<o:p></o:p>

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1<o:p></o:p>

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TECAN<o:p></o:p>

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Columbus<o:p></o:p>

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96<o:p></o:p>

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8/12<o:p></o:p>

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50-3000<o:p></o:p>

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＜2<o:p></o:p>

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1-24h<o:p></o:p>

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有<o:p></o:p>

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有<o:p></o:p>

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有<o:p></o:p>

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2<o:p></o:p>

</TD></TR></TBODY></TABLE>
 <o:p></o:p>

四、选购洗板机的注意事项

1、关键指标：残留量：＜2uL

能否提供可选择的洗板环境：洗板次数、条数、浸泡时间等是洗板机的基本参数，一般均可满足。建议选择具有底部冲洗、两点吸液等功能的仪器；底部冲洗有利于减小微孔板底部的干扰性吸附；两点吸液可大大降低微孔液体残留量。这对改进洗涤效果很有效。最小的残留量对PCR ELISA非常重要，因为在PCR ELISA操作中不允许拍板，以防污染。

2、是否对国产试剂盒具有兼容性与扩展性

兼容性主要是对于国内不同规格、尺寸略有差异的微孔板的兼容，仪器应具有对微孔板的位置调节功能，适应国内试剂的实际情况。扩展性主要是为了适应新出现的一些应用情况，如上海科华新增加的86式微孔板，许多洗板机无法清洗。

3、考察仪器的可靠性与洗涤效果的可靠性

一是仪器自身的可靠性，保证仪器在使用寿命期内可以正常显示、精确运动等，这可通过考查仪器生产厂商的技术与生产实力；一是洗涤效果的可靠性，即真正达到代替手工洗涤、甚至优于手工洗涤的目的，这需要咨询使用该仪器的上级单位或典型用户。

4、配件是否充分

厂方是否可以提供常用的、易损的配件，如清洗头、应该8道、12道各备用一个，并且定期淘汰，象洗液瓶、硅胶管等应方便买到。

5、售后服务是否及时、价格是否合理

洗板机是使用率相对较高的仪器，单从运动角度对比，其频率是酶标仪的几十倍，有的医院使用两年就报废，原因在于没有售后服务或售后服务价格太高，有的仅因为缺少一个配件而使整机报废，十分可惜。故及时的售后服务与相对合理的服务价格，是购买洗板机应考虑的因素。从保证洗板机效果的角度，建议用户根据使用情况，对洗板机进行定期的维护，而不是被动地等仪器不能工作时再去修理，这一工作可由厂家完成或在厂家协助下由有关人员完成。

6、人机界面是否友好，最好是中文显示

五、洗板机使用中注意事项

1、 有一定地洗涤次数：多次洗涤有利于取得好的效果，一般不超过6次。

2、 注意每孔的加液量：以加满为宜，但不可溢出，一般为每控300微升。

3、 建议在洗涤前进行位置调节，吸针要到微孔底部，以降低残留量。因为一般ELISA

实验洗涤结束后要求扣板，但此步骤在PCR-ELISA中易造成污染，故残留量应尽量减少。不能象一般ELISA操作一样用力扣板。

4、 建议用户采用两点吸液与底部冲洗方式，以得到好的洗涤效果。

5、 如果作PCR-ELISA液罐应加有1%次氯酸钠，加入的量不得少于产生废液的量。每

天使用完毕后应用1%次氯酸钠冲洗管路及清洗头，然后用双蒸水冲洗管路。

6、 定期维护、检修仪器及配件。

wildwolf 发表于 2004-3-31 00:00

洗板机使用范围

有谁知道，洗板机用在哪些方面？[em06]

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洗板机使用范围